虫草素对慢性肾脏病大鼠血管内皮损伤的保护作用及其机制

摘要 目的：探讨虫草素对慢性肾脏病(chronic kidney disease，CKD)大鼠血管内皮损伤的保护作用及其机制。方法：慢性肾脏病大鼠(n=48)随机平分为四组-模型组、虫草素高剂量组、虫草素中剂量组、虫草素低剂量组，虫草素高剂量组、虫草素中剂量组、虫草素低剂量组，分别给予虫草素160 mg/kg、80 mg/kg、40 mg/kg，模型组灌胃给予等量生理盐水，每天1次，连续给药治疗2周。结果：虫草素高剂量组、虫草素中剂量组、虫草素低剂量组治疗1周、治疗2周的24 h尿量、血清尿素氮(blood urea nitrogen，BUN)与肌酐(serum creatinine，Scr)水平都低于模型组(P<0.05)，体重都高于模型组(P<0.05)，不同剂量组别之间对比差异也有统计学意义(P<0.05)。虫草素高剂量组、虫草素中剂量组、虫草素低剂量组治疗2周的结缔组织生长因子(Connective tissue growth factor，CTGF)、血管内皮生长因子(Vascular endothelial growth factor，VEGF)蛋白相对表达水平高于模型组(P<0.05)，肾小球硬化指数、肾小管损伤评分都低于模型组(P<0.05)，不同剂量组别之间对比差异也有统计学意义(P<0.05)。结论：虫草素在慢性肾脏病大鼠的应用能发挥血管内皮损伤保护作用，促进改善大鼠的肾功能，提高大鼠的体重，且具有剂量依赖性。

Protective Effect of Cordycepin on Vascular Endothelial Injury in Rats with Chronic Kidney Disease and its Mechanism

ABSTRACT Objective: To investigate the protective effect of cordycepin on vascular endothelial injury in rats with chronic kidney disease (CKD) and its mechanism. Methods: Chronic kidney disease rats (n=48) were randomly equally divided into four groups-model group, cordycepin high-dose group, cordycepin middle-dose group, cordycepin low-dose group. The cordycepin high-dose group, cordycepin middle-dose group, cordycepin low-dose group were given 160 mg/kg, 80 mg/kg, 40 mg/kg cordycepin, and the model group were given the same amount of normal saline, once a day for 2 weeks. Results: The 24 h urine output, blood urea nitrogen(BUN) and serum creatinine (Scr) levels of cordycepin high-dose group, cordycepin middle-dose group and cordycepin low-dose group at 1 week and 2 weeks of treatment were all lower than the model group (P<0.05), and the body weight were higher than the model group (P<0.05). The difference compared among the different dose groups were also statistically significant(P<0.05). The relative expression levels of connective tissue growth factor(CTGF) and vascular endothelial growth factor(VEGF) proteins in the high-dose cordycepin group, the middle-dose cordycepin group, and the low-dose cordycepin group at 2 weeks of treatment were higher than the model group(P<0.05), the glomerular sclerosis index and renal tubular injury score were lower than the model group(P<0.05), and the difference compared among different dose groups were also statistically significant(P<0.05). Conclusion: The application of cordycepin in rats with chronic kidney disease can exert protective effect on vascular endothelial damage, promote the improvement of the renal function of rats, and increase the weight of rats, and it is dose-dependent.