自血疗法对慢阻肺稳定期大鼠治疗效果及对MMP-9和TIMP-1水平的影响

摘要 目的：分析自血疗法对慢阻肺稳定期大鼠治疗效果及对MMP-9和TIMP-1水平的影响。方法：将30只大鼠随机分成正常对照组、慢性阻塞性肺疾病（COPD）模型组和自血疗法组，每组10只。除正常对照组，其余大鼠采用熏香烟联合气道内灌注大肠杆菌内毒素建立慢阻肺稳定期大鼠模型。正常对照组和COPD模型组大鼠给予生理盐水治疗，自血疗法组给予穴位注射自体血液治疗。比较各组大鼠一般症状、肺功能、血清炎性因子、血清基质金属蛋白酶-9（MMP-9）和金属蛋白酶抑制剂-1（TIMP-1）水平以及肺组织中MMP-9和TIMP-1水平。结果：与正常对照组相比，COPD模型组大鼠的肺功能受损，体重、低0.3s用力呼气量（FEV0.3s）、用力肺活量（FVC）、FEV0.3s/ FVC（%）和呼气峰流速（PEF）均明显降低（P＜0.05），血清白介素-2（IL-2）、白介素-6（IL-6）、C反应蛋白（CRP）、降钙素原（PCT）、MMP-9和TIMP-1表达水平，呼吸频率以及肺组织中MMP-9和TIMP-1表达水平均明显升高（P＜0.05）。与COPD组相比，自血疗法组大鼠体重、FEV0.3s、FVC、FEV0.3s/ FVC（%）和PEV均明显升高（P＜0.05），血清IL-2、IL-6、CRP、PCT、MMP-9和TIMP-1表达水平，呼吸频率以及肺组织中MMP-9和TIMP-1表达水平均明显降低（P＜0.05）。结论：自血疗法可通过改善大鼠炎性反应程度，降低血清及肺组织中MMP-9和TIMP-1表达水平，达到改善或缓解大鼠肺慢阻症状的作用。

Therapeutic Effect of Autohemotherapy on Stable COPD Rats and Its Effect on the Levels of MMP-9 and TIMP-1

ABSTRACT Objective: To analyze the therapeutic effect of autohemotherapy on stable COPD rats and its effect on the levels of MMP-9 and TIMP-1. Methods: Thirty rats were randomly divided into normal control group, chronic obstructive pulmonary disease (COPD) model group and Autohemotherapy group, with 10 rats in each group. In addition to the normal control group, the rest of the rats were treated with cigarette smoking combined with E. coli endotoxin to establish stable COPD model. Rats in the normal control group and COPD model group were treated with normal saline, while rats in the Autohemotherapy group were treated with acupoint injection of autologous blood. The general symptoms, lung function, serum inflammatory factors, serum levels of matrix metalloproteinase-9 (MMP-9) and inhibitor of metalloproteinase-1 (TIMP-1) and the levels of MMP-9 and TIMP-1 in lung tissue were compared. Results: Compared with the normal control group, the lung function of COPD model group was impaired, such as body weight, forced expiratory volume (fev0.3s), forced vital capacity (FVC), fev0.3s/ FVC (%) and peak expiratory flow (PEF) were significantly decreased (P<0.05). The levels of serum interleukin-2 (IL-2), interleukin-6 (IL-6), C-reactive protein (CRP), procalcitonin (PCT), MMP-9 and TIMP-1 were significantly increased (P<0.05). Compared with the COPD group, the body weight, fev0.3s, FVC, fev0.3s / FVC (%) and PEV of the rats in the Autohemotherapy group were significantly increased (P<0.05), and the expression levels of IL-2, IL-6, CRP, PCT, MMP-9 and TIMP-1 in serum, respiratory rate and the expression levels of MMP-9 and TIMP-1 in lung tissue were significantly decreased (P<0.05). Conclusion: Autohemotherapy can improve or alleviate the symptoms of COPD by improving the degree of inflammatory reaction and reducing the expression levels of MMP-9 and TIMP-1 in serum and lung tissue.