毒毛花苷通过Nrf2抑制子宫内膜癌细胞增殖活性

摘要 目的：通过研究毒毛花苷对核因子2相关因子2 (nuclear factor erythroid 2-related factor 2, Nrf2)的抑制作用，深入探讨其抑制子宫内膜癌细胞增殖的作用机制。方法：（1）采用细胞增殖实验观察不同浓度的毒毛花苷对Ishikawa细胞增殖的影响；（2）通过克隆形成实验观察毒毛花苷对Ishikawa细胞增殖的作用；（3）采用蛋白免疫印迹法检测经毒毛花苷处理后，Ishikawa细胞Nrf2蛋白表达水平的变化。结果：经毒毛花苷处理后，Ishikawa细胞增殖受到显著抑制且呈剂量依赖性。Nrf2蛋白表达下调。结论：毒毛花苷可能通过下调Nrf2蛋白水平，从而抑制子宫内膜癌的恶性生物学行为。

Ouabain Inhibit the Proliferation of Endometrial Cells through Nrf2 Down-regulation

ABSTRACT Objective: By studying the inhibitory effect of Ouabain on nuclear factor erythroid 2-related factor 2 (Nrf2), the mechanism of how it suppresses endometrial cancer cell proliferation is deeply explored. Methods: (1) Cell proliferation experiments were used to observe the effect of Ouabain on the proliferation of Ishikawa cells; (2) Colony formation assays were used to observe the effect of Ouabain on the proliferation of Ishikawa cells; (3) Western blotting assays were used to detect the changes of Nrf2 protein level in Ishikawa cells after treatment with Ouabain. Results: After treatment with Ouabain, the proliferation of Ishikawa cells was markedly inhibited in a concentration dependent manner, and the expression of Nrf2 protein was down-regulated. Conclusion: Our finding suggested that Ouabain may inhibit the malignant biological behavior of endometrial cancer by down-regulating Nrf2.