神经节苷脂钠对缺血性脑卒中小鼠空间学习记忆能力的影响

摘要 目的：探讨神经节苷脂钠对缺血性脑卒中小鼠空间学习记忆能力的影响。方法：缺血性脑卒中小鼠模型(n=42)随机分为三组-模型组、氟西汀组与神经节苷脂钠组，每组14只小鼠。氟西汀组、神经节苷脂钠组、对照组分别给予10 mg/kg氟西汀与10 mg/kg神经节苷脂钠、等剂量生理盐水腹腔注射，1次/d，持续28 d。结果：氟西汀组、神经节苷脂钠组给药第7 d、14 d、28 d的逃避潜伏期、改良神经损伤严重程度评分(Modified neurological severity score，mNSS)低于模型组(P<0.05)，穿越平台次数高于模型组(P<0.05)，神经节苷脂钠组与氟西汀组对比差异有统计学意义(P<0.05)。氟西汀组、神经节苷脂钠组给药第28 d的海马组织B淋巴细胞瘤-2基因(B-cell lymphoma-2，Bcl-2)蛋白相对表达水平高于模型组(P<0.05)，BCL2-Associated X(Bax)蛋白相对表达水平、脑卒中相对面积低于模型组(P<0.05)，神经节苷脂钠组与氟西汀组对比差异有统计学意义(P<0.05)。结论：神经节苷脂钠在缺血性脑卒中小鼠的应用能促进恢复空间学习记忆能力，缓解神经损伤，抑制海马组织神经元细胞的凋亡，降低脑卒中面积。

Effects of Ganglioside Sodium on Spatial Learning and Memory in Mice with Ischemic Stroke

ABSTRACT Objective: To investigate the effects of ganglioside sodium on spatial learning and memory in mice with ischemic stroke. Methods: Mouse models of ischemic stroke(n=42) were randomly divided into three groups-model group, fluoxetine group and sodium ganglioside group, each groups with 14 cases. The fluoxetine group, the sodium ganglioside group, and the control group were given 10 mg/kg fluoxetine, 10 mg/kg sodium ganglioside, and the same dose of normal saline were injected intraperitoneally, once a day, for 28 days. Results: The escape latency and modified neurological severity score (mNSS) of the fluoxetine group and sodium ganglioside groupon the 7 th, 14 th, and 28 th day of administration were lower than the model group(P<0.05), the number of plateaus were higher than that of the model group (P<0.05), and compared the difference between the ganglioside sodium group and the fluoxetine group were statistically significant(P<0.05). The relative expression level of B-cell lymphoma-2(Bcl-2) protein in hippocampus on the 28 th day of administration in the fluoxetine group and the ganglioside sodium group were higher than the model group(P<0.05), and compared the difference between the ganglioside sodium group and the fluoxetine group were statistically significant(P<0.05). Conclusion: The application of sodium ganglioside in mice with ischemic stroke can promote the restoration of spatial learning and memory ability, relieve nerve damage, inhibit the apoptosis of hippocampal neurons and reduce the area of stroke.