血清免疫炎症相关蛋白复合物、25-羟维生素D、脂肪细胞因子与炎症性肠病患者疾病活动性和肠道菌群的相关性研究

摘要 目的：探讨血清免疫炎症相关蛋白复合物（IIRPCs）、25-羟维生素D[25（OH）D]、脂肪细胞因子（Chemerin）与炎症性肠病（IBD）患者疾病活动性和肠道菌群的相关性。方法：选取2020年12月～2021年12月我院收治的150例IBD患者，其中溃疡性结肠炎（UC）组65例、克罗恩病（CD）组85例，另取同期健康体检者70例作为对照组，检测并比较三组血清IIRPCs、25（OH）D、Chemerin水平。此外，UC组和CD组患者分别根据克罗恩病活动指数（CDAI）和溃疡性结肠炎的改良梅奥（Mayo）评分分为活动期组、缓解期组，分别比较UC组和CD组患者活动期组与缓解期组间的血清IIRPCs、25（OH）D、Chemerin水平、肠道菌群差异，并作相关性分析。结果：IBD患者的血清IIRPCs、Chemerin水平高于对照组，而25（OH）D水平低于对照组（P＜0.05）；UC组血清IIRPCs、Chemerin水平高于CD组，25（OH）D水平低于CD组（P＜0.05）。活动期UC、CD患者的血清IIRPCs、Chemerin水平以及肠球菌、肠杆菌、酵母菌、拟杆菌数量均高于缓解期UC、CD患者，而血清25（OH）D水平以及双歧杆菌、乳酸杆菌数量均低于缓解期UC、CD患者（P＜0.05）。Pearsonn相关性分析结果显示，UC、CD患者的血清IIRPCs、Chemerin水平与肠球菌、肠杆菌、酵母菌、拟杆菌数量呈正相关，与双歧杆菌、乳酸杆菌数量呈负相关（P＜0.05）；UC、CD患者的血清25（OH）D水平与肠球菌、肠杆菌、酵母菌、拟杆菌数量呈负相关，与双歧杆菌、乳酸杆菌数量呈正相关（P＜0.05）。结论：血清IIRPCs、25（OH）D、Chemerin与IBD患者的疾病活动性、肠道菌群有关，检测上述指标对评估IBD患者病情程度有一定价值。

Correlation Study of Serum Immune Inflammation Related Protein Complexes, 25-Hydroxyvitamin D, Chemerin with Disease Activity and Intestinal Flora in Patients with Inflammatory Bowel Disease

ABSTRACT Objective: To investigate the correlation between serum immune inflammation related protein complexes (IIRPCs), 25-hydroxyvitamin D [25(OH)D], Chemerin (Chemerin) and disease activity and intestinal flora in patients with inflammatory bowel disease (IBD). Methods: 150 patients with IBD who were treated in our hospital from December 2020 to December 2021 were selected, including 65 cases of ulcerative colitis (UC) group and 85 cases of Crohn''s disease (CD) group, and 70 healthy subjects in the same period were taken as the control group. The levels of serum IIRPCs, 25(OH)D and Chemerin in the three groups were detected and compared. In addition, patients in UC group and CD group were divided into active group and remission group according to the Crohn''s disease activity index (CDAI) and the modified Mayo score of ulcerative colitis. The levels of serum IIRPCs, 25(OH)D, Chemerin and intestinal flora between active group and remission group were compared, and correlation analysis was made. Results: The levels of serum IIRPCs and Chemerin in patients with IBD were higher than those in the control group, while the level of 25(OH)D was lower than that in the control group (P<0.05). The levels of serum IIRPCs and Chemerin in UC group were higher than those in CD group, and the level of 25(OH)D was lower than that in CD group (P<0.05). The levels of serum IIRPCs, Chemerin, Enterococcus, enterobacter, yeast and bacteroides in patients with active UC and CD were higher than those in patients with remission UC and CD, while the levels of serum 25(OH)D, bifidobacteria and lactobacillus were lower than those in patients with remission UC and CD (P<0.05). Pearsonn correlation analysis showed that the levels of serum IIRPCs and Chemerin in patients with UC and CD were positively correlated with the number of Enterococcus, enterobacter, yeast and Bacteroides, and negatively correlated with the number of Bifidobacterium and Lactobacillus (P<0.05). The level of serum 25(OH)D in patients with UC and CD was negatively correlated with the number of Enterococcus, enterobacter, yeast and Bacteroides, and positively correlated with the number of Bifidobacterium and Lactobacillus (P<0.05). Conclusion: Serum IIRPCs, 25(OH)D and Chemerin are related to disease activity and intestinal flora in patients with IBD. The detection of the above indicators is of certain value in evaluating the severity of patients with IBD.