子宫内膜癌组织KIF20A、LAPTM4B-35表达与临床病理特征及预后的关系研究

摘要 目的：探讨子宫内膜癌组织驱动蛋白家族成员20A(KIF20A)、溶酶体相关4次跨膜蛋白B-35(LAPTM4B-35)表达与临床病理特征及预后的关系。方法：选择2012年4月至2015年8月期间于我院行手术治疗的80例子宫内膜癌患者作为研究对象。检测子宫内膜癌组织以及距离肿瘤边缘2 cm以上癌旁组织中KIF20A、LAPTM4B-35 mRNA表达水平。分析子宫内膜癌组织中KIF20A、LAPTM4B-35 mRNA表达与临床病理特征的关系。分析不同KIF20A、LAPTM4B-35 mRNA表达患者5年总生存率的差异。分析子宫内膜癌患者预后的影响因素。结果：与癌旁组织相比，子宫内膜癌组织中KIF20A、LAPTM4B-35 mRNA表达水平明显升高，差异有统计学意义（P<0.05）。有淋巴结转移以及FIGO分期Ⅲ期患者的癌组织KIF20A、LAPTM4B-35 mRNA表达水平明显高于无淋巴结转移以及FIGO分期I～II期患者的癌组织，差异有统计学意义（P<0.05）。KIF20A低表达组患者5年总生存率明显高于KIF20A高表达组；LAPTM4B-35低表达组患者5年总生存率明显高于LAPTM4B-35高表达组患者，差异有统计学意义（P<0.05）。Cox回归分析结果显示：FIGO分期Ⅲ期、有淋巴结转移、KIF20A mRNA高表达和LAPTM4B-35 mRNA高表达是子宫内膜癌患者预后的影响因素（P<0.05）。结论：在子宫内膜癌组织中KIF20A、LAPTM4B-35 mRNA表达水平升高，有淋巴结转移、FIGO分期较高患者癌组织KIF20A、LAPTM4B-35 mRNA表达水平上调。KIF20A 、LAPTM4B-35高表达患者5年总生存率下降。

Relationship Study between the Expression of KIF20A and LAPTM4B-35 and Clinicopathological Features and Prognosis in Endometrial Carcinoma

ABSTRACT Objective: To investigate the relationship between the expression of kinesin family member 20A (KIF20A) and lysosome-associated protein transmembrane 4 beta-35 (LAPTM4B-35) and clinicopathological features and prognosis in endometrial carcinoma. Methods: 80 patients with endometrial carcinoma who underwent surgery in our hospital from April 2012 to August 2015 were selected as the research objects. The expression levels of KIF20A and LAPTM4B-35 mRNA in endometrial carcinoma tissues and paracancerous tissue more than 2cm away from the tumor edge were detected. The relationship between the expression of KIF20A, LAPTM4B-35 mRNA and clinicopathological features in endometrial carcinoma were analyzed. The difference of 5-year overall survival rate in patients with different KIF20A and LAPTM4B-35 mRNA expression was analyzed. The prognostic factors of patients with endometrial carcinoma were analyzed. Results: Compared with the paracancerous tissue, the expression levels of KIF20A and LAPTM4B-35 mRNA in endometrial carcinoma tissues were significantly increased, and the differences were statistically significant (P<0.05). The expression levels of KIF20A and LAPTM4B-35 mRNA in carcinoma tissues of patients with lymph node metastasis and FIGO stage III were significantly higher than those of patients without lymph node metastasis and FIGO stage I ~ II, with statistical significance(P<0.05). The 5-year overall survival rate of patients with low KIF20A expression group was significantly higher than that of patients with high KIF20A expression group. The 5-year overall survival rate of patients with low LAPTM4B-35 expression group was significantly higher than that of patients with high LAPTM4B-35 expression group, with statistical significance(P<0.05). Cox regression analysis showed that FIGO stage III, lymph node metastasis, high expression of KIF20A mRNA and LAPTM4B-35 mRNA were the prognostic factors of patients with endometrial carcinoma(P<0.05). Conclusion: The expression levels of KIF20A and LAPTM4B-35 mRNA in endometrial carcinoma tissues are increased, and the expression levels of KIF20A and LAPTM4B-35 mRNA in carcinoma tissues of patients with lymph node metastasis and higher FIGO stage are increased. The 5-year overall survival rate of patients with high expression of KIF20A and LAPTM4B-35 decreased.