Derivation of Induced Pluripotent Stem Cells from Human Peripheral Blood T Lymphocytes |
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Authors: | Matthew E. Brown Elizabeth Rondon Deepika Rajesh Amanda Mack Rachel Lewis Xuezhu Feng Laura Jo Zitur Randall D. Learish Emile F. Nuwaysir |
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Affiliation: | Department of Research and Development, Cellular Dynamics International, Inc., Madison, Wisconsin, United States of America.;New York University, United States of America |
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Abstract: | Induced pluripotent stem cells (iPSCs) hold enormous potential for the development of personalized in vitro disease models, genomic health analyses, and autologous cell therapy. Here we describe the generation of T lymphocyte-derived iPSCs from small, clinically advantageous volumes of non-mobilized peripheral blood. These T-cell derived iPSCs (“TiPS”) retain a normal karyotype and genetic identity to the donor. They share common characteristics with human embryonic stem cells (hESCs) with respect to morphology, pluripotency-associated marker expression and capacity to generate neurons, cardiomyocytes, and hematopoietic progenitor cells. Additionally, they retain their characteristic T-cell receptor (TCR) gene rearrangements, a property which could be exploited for iPSC clone tracking and T-cell development studies. Reprogramming T-cells procured in a minimally invasive manner can be used to characterize and expand donor specific iPSCs, and control their differentiation into specific lineages. |
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