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Dynamic cell surface hydrophobicity of Lactobacillus strains with and without surface layer proteins
Authors:Vadillo-Rodríguez Virginia  Busscher Henk J  Norde Willem  de Vries Joop  van der Mei Henny C
Affiliation:Department of Biomedical Engineering, University of Groningen, Groningen, The Netherlands.
Abstract:Variations in surface hydrophobicity of six Lactobacillus strains with and without an S-layer upon changes in ionic strength are derived from contact angle measurements with low- and high-ionic-strength aqueous solutions. Cell surface hydrophobicity changed in response to changes in ionic strength in three out of the six strains, offering these strains a versatile mechanism to adhere to different surfaces. The dynamic behavior of the cell surface hydrophobicity could be confirmed for two selected strains by measuring the interaction force between hydrophobic and hydrophilic tips with use of atomic force microscopy.
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