The Putative Lipase,AF1763, from <Emphasis Type="Italic">Archaeoglobus fulgidus</Emphasis>is a Carboxylesterase with a Very High pH Optimum |
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Authors: | Monika?Rusnak Jens?Nieveler Rolf?D?Schmid Email author" target="_blank">Ralf?PetriEmail author |
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Institution: | (1) Institute for Technical Biochemistry, University of Stuttgart, Allmandring 31, 70569 Stuttgart, Germany;(2) Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Cologne, Germany |
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Abstract: | The open reading frame AF1763, annotated as a putative lipase gene (lipA) of the
hyperthermophilic archaeon, Archaeoglobus fulgidus
DSM 4304, was cloned and over-expressed in E. coli.
A sequence analysis of LipA and the investigation of a truncated enzyme implied a special
function of the C-terminal part of LipA.
The substrate spectrum of the enzyme suggested that LipA is a carboxylesterase rather than a
canonical lipase. The enzyme showed optimal activity at 70 °C and between pH 10
and 11, which is among the most alkaline pH range detected for hydrolases. |
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Keywords: | Archaeoglobus fulgidus carboxylesterase pH stability |
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