Introduction of 5'-terminal functional groups into synthetic oligonucleotides for selective immobilization |
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Authors: | R Bischoff J M Coull F E Regnier |
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Institution: | Department of Biochemistry, Purdue University, West Lafayette, Indiana 47907. |
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Abstract: | Oligodeoxyribonucleotides terminating in a 5'-primary amine group are synthesized using solid-phase supported phosphoramidite chemistry. The 5'-terminal amine group in the deprotected oligomers is further derivatized with either succinic anhydride to give 5'-carboxylic acid or with dithiobis(succinimidylpropionate) followed by treatment with dithioerythritol to produce 5'-thiol-terminated oligonucleotides. The 5'-thiol-terminated oligonucleotides are selectively immobilized on solid supports containing either p-chloromercuribenzoate or 2,2'-dithiobis(5-nitropyridine) activated thiol groups. |
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