HMG-proteins 1 and 2 are required for transcription of chromatin by endogenous RNA polymerase |
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| Authors: | J A Stoute W F Marzluff |
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| Affiliation: | Department of Biochemistry II, Division of Chemical Endocrinology, Erasmus University Rotterdam, P.O. Box 1738, 3000 DR Rotterdam, The Netherlands |
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| Abstract: | An androgen receptor was isolated and partially purified from the cytosol fraction of ram seminal vesicles. An almost two thousand fold purification with a recovery of 33% could be obtained by chromatography on 2′,5′-ADP-sepharose, ammoniumsulphate precipitation and gel filtration on Ultrogel ACA-44. The labelled receptor was characterized by electrophoresis and sucrose gradient centrifugation (sedimentation at approximately 3S). The purified receptor has a DNA binding-site and is specific for androgenic steroids. |
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