Mitochondrial DNA analysis of Exophiala jeanselmei var. lecanii-corni and Exophiala castellanii |
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| Authors: | Kawasaki Masako Ishizaki Hiroshi Matsumoto Tadahiko Matsuda Tetsuo Nishimura Kazuko Miyaji Makoto |
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| Institution: | (1) Department of Dermatology, Kanazawa Medical University, Ishikawa, Japan;(2) Toshiba Hospital, Tokyo, Japan;(3) Department of Dermatology, Kyushu University, Fukuoka, Japan;(4) Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, Chiba, Japan |
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| Abstract: | A Japanese clinical isolate (KU-A-0094) which was identified by de Hoog et al. as Exophiala jeanselmei var. lecanii-corni with difficulty, was compared with 5 strains including the type cultures of E. jeanselmei var. lecanii-corni, var. jeanselmei and E. castellanii using RFLP (restriction fragment length polymorphism) patterns of mtDNA (mitochondrial DNA). RFLP patterns of KUA-0094 were
identical with those of E. jeanselmei var. lecanii-corni and different from those of E. castellanii with restriction enzymes of HaeIII, MspI and hindIII. Therefore, de Hoog et al.'s identification of KU-A-0094 was confirmed. Additionally, mtDNA-RFLP patterns of E. jeanselmei var. lecanii-corni and E. jeanselmei var. jeanselmei were also different from each other. Consequently E. jeanselmei var. lecanii-corni seem to be a species in its own right rather than a variant of E. jeanselmei.
This revised version was published online in June 2006 with corrections to the Cover Date. |
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| Keywords: | E castellanii E jeanselmei var jeanselmei E jeanselmei var lecanii-corni Mitochondrial DNA RFLP pattern |
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