Induction of aromatic ring: cleavage dioxygenases in <Emphasis Type="Italic">Stenotrophomonas maltophilia</Emphasis> strain KB2 in cometabolic systems |
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Authors: | Danuta Wojcieszyńska Urszula Guzik Izabela Greń Magdalena Perkosz Katarzyna Hupert-Kocurek |
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Institution: | (1) Faculty of Biology and Environment Protection, Department of Biochemistry, University of Silesia, Jagiellonska 28, 40-032 Katowice, Poland; |
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Abstract: | Stenotrophomonas maltophilia KB2 is known to produce different enzymes of dioxygenase family. The aim of our studies was to determine activity of these
enzymes after induction by benzoic acids in cometabolic systems with nitrophenols. We have shown that under cometabolic conditions
KB2 strain degraded 0.25–0.4 mM of nitrophenols after 14 days of incubation. Simultaneously degradation of 3 mM of growth
substrate during 1–3 days was observed depending on substrate as well as cometabolite used. From cometabolic systems with
nitrophenols as cometabolites and 3,4-dihydroxybenzoate as a growth substrate, dioxygenases with the highest activity of protocatechuate
3,4-dioxygenase were isolated. Activity of catechol 1,2- dioxygenase and protocatechuate 4,5-dioxygenase was not observed.
Catechol 2,3-dioxygenase was active only in cultures with 4-nitrophenol. Ability of KB2 strain to induce and synthesize various
dioxygenases depending on substrate present in medium makes this strain useful in bioremediation of sites contaminated with
different aromatic compounds. |
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