Organ culture of human nervous system tumors |
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| Authors: | Katherine M. Lyser |
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| Affiliation: | (1) Institut d'Embryologie Expérimentale du Collège de France et du Centre Nationale de la Recherche Scientifique, 49 bis, Avenue de la Belle-Gabrielle, 94130 Nogent-sur-Marne, France;(2) Present address: Hunter College, 695 Park Avenue, Box 1030, 10021 New York, New York;(3) Department of Biological Sciences, Hunter College of the City University of New York, New York, USA |
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| Abstract: | Summary Tumors of the human nervous system (neuroblastomas, an ependymoma, a medulloblastoma, and a Schwannoma) obtained during surgery have been cultured organotypically by the method of Wolff. The tumors retained characteristic morphology, organization and patterns of behavior in vitro, and one neuroblastoma gave rise to a growing long-term culture. Long-term organotypic culture, where maintenance of tissue organization and growth occur together, is recommended for the study of neoplasms of the nervous system. |
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| Keywords: | neuroblastomas nervous system tumors organ culture differentiation |
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