Defined megadalton hyaluronan polymer standards |
| |
Authors: | Jing Wei Haller F Michael Almond Andrew DeAngelis Paul L |
| |
Institution: | Hyalose LLC, Oklahoma City, OK 73104, USA. |
| |
Abstract: | The utility of polymer standards for the calibration of average molecular mass estimates often is limited by the polydispersity--the breadth of the size distribution--of the standard. Here monodisperse synthetic hyaluronan (or hyaluronic acid HA]) complexes in the approximately 1- to 8-megadalton (MDa) range were prepared in two steps. First, synchronized stoichiometrically controlled in vitro reactions yielded linear narrow size distribution biotinylated HA chains. Second, streptavidin protein was added at substoichiometric levels to prepare a series of complexes with one, two, three, or four HA chains per streptavidin molecule. The dendritic-like molecules approximate the mobility of natural linear HA chains on agarose gels, making the complexes useful as defined size standards for high-molecular weight HA preparations. |
| |
Keywords: | Glycosaminoglycan Hyaluronic acid Synthase Monodisperse Polysaccharide Pasteurella Biotin Avidin |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|