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Optimization of growth and jaceosidin production in callus and cell suspension cultures of Saussurea medusa
Authors:Zhao  Dexiu  Xing  Jianming  Li  Maoyin  Lu  Dongping  Zhao  Qiao
Affiliation:(1) Institute of Botany, Chinese Academy of Sciences, Beijing, 100093, China;(2) China Agricultural University, 100094, China
Abstract:Calli cultures derived from the leaves of Saussurea medusa were selected on the basis of colour into three callus, A, B and C, which suggested different levels of metabolite accumulation. An improved reversed phase high performance liquid chromatographic method provided selective determination of the jaceosidin content of these samples. The jaceosidin concentration in callus B was higher than that of the callus A and C. By using 12-day old culture and 9-day old inoculum, jaceosidin yield of 72.91 mg l–1was obtained from cell line B in cell suspension cultures. The influence of some factors affecting jaceosidin formation, i.e. temperature, light, inoculum size, type of media, phytohormones, nitrogen and carbon source etc. were also examined. Light irradiation and combination of 3% (w/v) sucrose with 1% glucose brought about a marked increase of jaceosidin production. The effect of blue light on jaceosidin was markedly superior to other kinds of monochromatic light (red and far-red) or white light. Analysis of growth and jaceosidin content of callus cultures and cell suspension cultures demonstrated that the production of jaceosidin was growth-dependent in both cell solid culture and cell suspension culture.
Keywords:high performance liquid chromatography  jaceosidin  plant cell culture  Saussurea medusa
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