Studies on melon necrotic spot virus disease of cucumber and on the control of the fungus vector (Olpidium radicale) |
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Authors: | J A TOMLINSON B J THOMAS |
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Institution: | National Vegetable Research Station, Wellesbourne, Warwick, CV35 9EF;Glasshouse Crops Research Institute, Littlehampton, Sussex, BN17 6LP |
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Abstract: | Melon necrotic leaf spot virus (MNSV) caused a major outbreak of a leaf necrosis disease of hydroponically-grown cucumber plants at Humberside in 1983. The virus had c. 33 nm diam. particles which reacted serologically with MNSV antiserum of Dutch or American origin. Virus particles, which contained a single polypeptide (mol. wt 45 × 103) and a presumed RNA species (mol. wt 1.5 × 106), had a sedimentation coefficient (s20.w) of 134 S and a buoyant density in caesium chloride of 1.35 g/cm3. The virus was mechanically transmissible, confined to species of Cucurbitaceae, transmitted by zoospores of Olpidium radicale and retained in the resting spores of the fungus. MNSV is thus both water-borne and soil-borne. O. radicale zoospores were killed in <5 min in suspensions containing 20 μg/ml of the surfactant Agral (alkyl phenol ethylene oxide). The disease did not reappear in 1984 when the cucumber crops were fed with nutrients containing 20μg/ml Agral. |
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