HCf-6, a novel class II hydrophobin from Cladosporium fulvum |
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Authors: | Nielsen P S Clark A J Oliver R P Huber M Spanu P D |
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Institution: | Carlsberg Laboratory, Department of Yeast Genetics, Copenhagen Valby, Denmark. |
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Abstract: | C. fulvum, a fungal tomato pathogen, has previously been shown to express a complex family of hydrophobin genes including four class I hydrophobins and one class II hydrophobin. Here we describe a gene for HCf-6, a sixth member of the hydrophobin family and the second class II gene. The protein is predicted to consist of a signal sequence, an N-terminus rich in glycine and asparagine and a C-terminal hydrophobic domain which bears the hall-marks of hydrophobins. In contrast to the previously described class II hydrophobin HCf-5, HCf-6 is expressed in mycelium growing in pure culture and mRNA levels do not increase during sporulation. It is down-regulated by carbon starvation but not by depletion of nitrogen in the growth medium. |
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Keywords: | Hydrophobins Cladosporium fulvum sporulation glycine/asparagine-rich tomato leaf mould |
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