High-level expression of recombinant active human renin in Sf-9 cells: rapid purification and characterization |
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| Authors: | Takahashi Saori Hata Keishi Kikuchi Ken-Ichi Gotoh Takeshi |
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| Institution: | Institute for Food and Brewing, Akita Prefectural Agricultural, Forestry, and Fisheries Research Center, Japan. saori@arif.pref.akita.jp |
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| Abstract: | Recombinant human (rh) renin was expressed in Sf-9 insect cells. Baculovirus-infected Sf-9 cells produced active rh-renin in the late stage of cultivation. The rh-renin was purified after 5 d of culture by two steps of column chromatography. Approximately 0.61 mg of pure rh-renin was obtained from 200 ml of culture medium with a yield of 35.3%. |
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