In vivo chlorophyll forms in higher plants and algae sensitive to treatment with lutein |
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Authors: | Konishi Katsuko; Ogawa Teruo; Inoue Yorinao; Shibata Kazuo |
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Institution: | 1Laboratory of Biological Chemistry, Tokyo Institute of Technology Meguroku, Tokyo
2Laboratory of Plant Physiology, Institute of Physical and Chemical Reserach (Rikagaku Kenkyusho) Wako-shi, Saitama, Japan |
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Abstract: | Lamella preparations of spinach, Chlorella, Phaeodactylum, Anabatnaand Porphyra were treated with a hydrophobic reagent, lutein,and the absorption and fluorescence spectra in the red regionbefore and after treatment were compared for changes causedby the treatment. Absorption spectra of all these preparationsunderwent the same spectral change, transformation of a bandat 684 nm into a band at 666 nm. The longer the maximum wavelengthof the red peak, the greater was the fractional absorbance decreaseat 684 nm. The content of C684 (the chlorophyll form responsiblefor the 684 nm band) in the lamellae was estimated from thefractional decreases as being progressively higher in the orderof Phaeodactylum, Porphyra, Anabatna, Chlorella and spinach.The fluorescence spectra at liquid nitrogen temperature beforetreatment showed two bands. The longer wavelength band was transformedby the treatment into a shorter wavelength band(s), as describedbelow, according to the maximum wavelengths: spinach, F735 F695(or F686); Chlorella, F715 F700 (or F686); Phaeodactylum, anunidentified component F690; Anabaena, F732 F685 (or F695); Porphyra,F726 F683. These chlorophyll forms with fluorescence maxima between715 and 735 nm were, therefore, designated C684 based on absorptionspectrophotometry, and are considered to play a role in photosystemII. (Received August 15, 1972; ) |
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