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Assessment of Keratinase and Other Hydrolytic Enzymes in Thermophilic Bacteria Isolated from Geothermal Areas in Patagonia Argentina
Authors:I Cavello  M S Urbieta  A B Segretin  A Giaveno  S Cavalitto  E R Donati
Institution:1. CINDEFI (CCT La Plata-CONICET, UNLP), Facultad de Ciencias Exactas, Universidad Nacional de La Plata, La Plata, Argentina;2. PROBIEN (CONICET-UNCo), Laboratorio de Biolixiviación, Departamento de Química - Facultad de Ingeniería, Universidad Nacional del Comahue, Neuquén, Argentina
Abstract:Thermophilic aerobic bacteria were isolated from two geothermal areas in Neuquén province using two different enrichment methods and a total of 30 isolates were obtained. From chicken feather enrichment cultures, strains affiliated to Bacillus cytotoxicus and Bacillus licheniformis were isolated and all of them demonstrated the capability to degrade completely chicken feather. A preliminary research on biotechnological enzymes' potential demonstrated that all the isolates displayed at least one of the extracellular hydrolytic enzymes tested. Most of the isolates showed protease, inulinase and/or pectinase activities, while cellulase and xylanase activities were less common. In light of these findings, geothermal areas of Argentina may be considered as a potential source of thermophilic bacteria able to produce many industrially relevant enzymes.
Keywords:Biodiversity  keratinolytic microorganisms  thermophilic bacteria
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