Culture of chlorophyllous tobacco-cells not requiring any organic additives except sucrose in the medium II. Mineral accumulation by the cells

1. There appeared to be a high degree of constancy in the nutrientcontents in NG (Nicotiana glutinosa) cells where the mediumcomposition remained stable, while the nutrient contents inHNG (habituated NG) cells varied with the changes in the compositionof the medium during the culture period of 30 days.
2. The respectivenutrient-content ratios of HNG to NG cells were:N (1.06)Ca(l.08)<Mg (1.26)<K (2.06)<P (3.57) in theorder ofmagnitude on the 5th day from the start of culture,when bothmedia compositions of NG and HNG cells were stillunchanged.

During this culture period, contents of Mg, K and P were markedlyhigher in HNG than in NG cells, and nutrient contents were generallyhigher in HNG than in NG cells. (Received January 17, 1978; )