Immunological evidence to show that the N-acetylglucosaminidase and N-acetylgalactosaminidase activities of Dictyostelium discoideum reside in the same protein molecule (Short Communication) |
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Authors: | D Every and J M Ashworth |
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Institution: | Department of Biochemistry, School of Biological Sciences, University of Leicester, Leicester LE1 7RH, U.K. |
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Abstract: | Antibodies were produced against purified beta-N-acetylhexosaminidase from Dictyostelium discoideum. Ouchterlony tests produced a single precipitin band with both beta-N-acetylglucosaminidase and beta-N-acetylgalactosaminidase activities. Both activities were co-precipitated in exactly the same proportions in quantitative immunoprecipitation reactions. We conclude that one enzyme protein catalyses the hydrolysis of both N-acetylglucosaminides and N-acetylgalactosaminides. |
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