Illumination of Arabidopsis roots induces immediate burst of ROS production |
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| Authors: | Ken Yokawa Tomoko Kagenishi Tomonori Kawano Stefano Mancuso Franti?ek Balu?ka |
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| Institution: | 1.Laboratory of Chemical Biology and Environmental Engineering; Faculty and Graduate School of Environmental Engineering; University of Kitakyushu; Kitakyushu, Japan;2.IZMB; University of Bonn; Bonn, Germany;3.LINV; Plant, Soil & Environmental Science; University of Firenze; Sesto Fiorentino, Italy |
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| Abstract: | Arabidopsis roots are routinely exposed to light both during their cultivation within transparent Petri dishes and during their confocal microscopy analysis. Here we report that illumination of roots which naturally grow in darkness, even for a few seconds, induces an immediate and strong burst of reactive oxygen species (ROS). Plant scientists studying roots should pay great attention to the environment of living roots, and keep them in darkness as long as possible. Results obtained using illuminated roots during in vivo microscopic analysis should also be interpreted with great caution.Key words: reactive oxygen species, root, light, superoxide, escape phototropism |
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