Decreased ribonucleic acid synthesis in isolated rat liver nuclei during starvation |
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| Authors: | D Rickwood and H G Klemperer |
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| Institution: | Department of Biochemistry, University of Birmingham, Birmingham 15, U.K. |
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| Abstract: | 1. Isolated nuclei from starved rats showed a lowered incorporation of (14)C]UMP into RNA. 2. The Mg(2+)-dependent incorporation was decreased by 30% after 1 day of starvation, but incorporation in the presence of Mn(2+) and ammonium sulphate decreased only after longer periods of starvation. 3. RNA synthesis by nuclei in the presence of excess of added RNA polymerase was unchanged after 1 day of starvation and was inhibited by 20% after 4 days. 4. The capacity of nuclei to bind actinomycin D was unchanged after 1 day and was decreased by 20% after 4 days of starvation. |
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