Characterisation of RC-proteoliposomes at different RC/lipid ratios |
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Authors: | Francesco Milano Francesca Italiano Angela Agostiano Massimo Trotta |
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Institution: | (1) CNR, Istituto per i Processi Chimico-Fisici, Sezione di Bari, c/o Dipartimento di Chimica, Via Orabona, 4 I-70124 Bari, Italy;(2) Dipartimento di Chimica, Università degli studi di Bari, Via Orabona, 4 I-70124 Bari, Italy |
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Abstract: | Reconstitution of membrane proteins in phospholipid vesicles allows the investigation of such macromolecules in a biomimetic
simplified environment. The often employed micelle-to-vesicle-transition method for proteoliposome preparation is a fast and
reproducible technique. In this, communication is shown that the lipid/protein ratio influences the size of the proteoliposomes
and the actual protein reconstitution. The results indicate that for photosynthetic reaction centres, the best conditions
for ligand-interaction experiments are achieved with a lipid/protein value of 1000:1, while for complete protein incorporation,
the 2000:1 ratio should be chosen. |
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Keywords: | Liposomes Reconstitution Micelle-to-vesicle-transition Size exclusion chromatography Dynamic light scattering |
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