Mycobacterium indicus pranii supernatant induces apoptotic cell death in mouse peritoneal macrophages in vitro |
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Authors: | Pandey Rajeev Kumar Bhatt Kunal H Dahiya Yogesh Sodhi Ajit |
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Institution: | School of Biotechnology, Faculty of Science, Banaras Hindu University, Varanasi, India. |
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Abstract: | Mycobacterium indicus pranii (MIP), also known as Mw, is a saprophytic, non-pathogenic strain of Mycobacterium and is commercially available as a heat-killed vaccine for leprosy and recently tuberculosis (TB) as part of MDT. In this study we provide evidence that cell-free supernatant collected from original MIP suspension induces rapid and enhanced apoptosis in mouse peritoneal macrophages in vitro. It is demonstrated that the MIP cell-free supernatant induced apoptosis is mitochondria-mediated and caspase independent and involves mitochondrial translocation of Bax and subsequent release of AIF and cytochrome c from the mitochondria. Experiments with pharmacological inhibitors suggest a possible role of PKC in mitochondria-mediated apoptosis of macrophages. |
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