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Heterogeneity of Maize Root Mitochondria from Plants Grown in the Presence of Ammonium
Authors:V. Hadži-Tašković Šukalović  M. Vuletić
Affiliation:(1) Laboratory of Plant Physiology, Maize Research Institute, Zemun Polje, P.O. Box 89, 11081 Beograd-Zemun, Yugoslavia
Abstract:Mitochondria isolated from root tissue of maize plants grown on a modified Knop solution containing 10.9 mM nitrate ± 7.2 mM ammonium were purified on the discontinuous Percoll density gradient with polyvinylpyrrolidone (PVP) added. The presence of PVP allowed separation of several mitochondrial fractions of a different density. Contrary to mitochondria isolated from plants grown in the presence of nitrate alone, revealing only two fractions, the mitochondria from NH4+/NO3-plants were distributed in four fractions. Total amount of mitochondria, as well as specific activities of some nitrogen metabolism enzymes and tricarboxylic acid (TCA) cycle enzymes of all mitochondrial fractions, and respiratory activities of two lower density fractions isolated from plants grown on mixed nitrogen were higher in comparison to mitochondria from nitrate-grown plants.
Keywords:nitrogen form  respiration  Zea mays L.
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