转基因动物研究新进展

我们曾对转基因动物的制作方法、转基因动物研究的应用、转基因的表达特征及提高转基因表达的策略等作过专题综述＾「１」。而这之后在转基因动物的研究方面又获得了许多新进展，其中转线粒体动物的问世拓宽了转基因动物的研究内容。在制作转基因动物的方法上，１９９８－１９９９年世界上先后报道了三种新方法：即由Ｓｃｈｎｉｅｋ等报道的体细胞核移植技术实现转基因，由Ａｎｔｈｏｎｙ Ｗ．Ｓ．Ｃ等报道的通过用逆转录病毒载体感

New Progress in Animal Transgenic Research

Some ways of animal transgenic research, such as methodology of animal transgenic makings, application of transgenic research, expression characteristics of transgenes in the genome of transgeic animals and the strategies to improve the expression of transgenes were reviewed by us. After that some new progress had been made in the following years, including the invention of mitochondrial transfer techniques, which brodened the fields of animal transgenic research. As far as the methods for animal transgenic making were concerned, three new methods for animal transgemic making were reported during 1998 and 1999, including gene transfer by nuclear transfer of animal somatic cells, reverse-transcribed gene transfer and intracytoplasmic sperm injection. When talking about the application of animal transgenic research, rat intestinal lactase-phlorizin hydrolase cDNA was integrated into the mouse genome, and lactose in the milk of transgenic mice was reduced by 50% to 85%. There were also some new reports on the explanation of low level expression of transgenes in the genome of their hosts.