Physicochemical surface properties ofKlebsiella pneumoniae |
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Authors: | Silvia Camprubí Susana Merino Javier Benedí Paul Williams Dr Juan M Tomás |
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Institution: | (1) Department of Microbiology, University of Barcelona, 08071 Barcelona, Spain;(2) Laboratory of Microbiology, University of Islas Baleares, Palma de mallorca, Spain;(3) Department of Microbiology, School of Pharmaceutical Sciences, University of Nottingham, Nottingham, UK |
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Abstract: | The following cell surface physicochemical characteristics were investigated inKlebsiella pneumoniae: surface charge, surface hydrophobicity by different methods, and accessibility of the lipid fraction of the outer membrane. The capsular polysaccharide, as well as the O-antigen repeating units of the lipopolysaccharide (LPS), conferred a hydrophilic, negatively charged surface to the bacterium, and a barrier to the dye congo red, which binds sites within the lipid fraction of the outer membrane (OM). |
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