Automation of MLST using third-generation liquid-handling technology |
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| Authors: | C B Sullivan J M C Jefferies M A Diggle S C Clarke |
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| Institution: | (1) Scottish Meningococcus and Pneumococcus Reference Laboratory, Glasgow, UK;(2) Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK;(3) Improving Health and Quality Directorate, Portsmouth City Primary Care Trust, Finchdean House, Milton Road, PO3 6DP Portsmouth, UK |
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| Abstract: | The molecular characterization of bacterial pathogens of clinical significance is increasingly important. Methods, such as
multilocus sequence typing (MLST), allow bacterial strains to be characterized during case clusters, for antibiotic-resistant
strains to be monitored, and for the impact of new vaccines to be assessed. Our laboratory performs MLST on Neisseria meningitidis, Streptococcus pneumonaie, Haemophilus influenzae, and Staphylococcus aureus. We have developed high-throughput automated methods to allow MLST to be performed in a time scale useful in a clinical setting.
Here we describe the automation of MLST on a third-generation liquid-handling robot. |
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| Keywords: | Automation MLST meningococci pneumococci typing molecular characterization |
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