Cell death evaluation in benzo[a]pyrene-transformed human breast epithelial cells after microcell-mediated transfer of chromosomes 11 and 17 |
| |
Authors: | Mello Maria Luiza S Barbisan Luis Fernando Lareef Mohamed H Russo Jose Vidal Benedicto de Campos |
| |
Affiliation: | Department of Cell Biology, Institute of Biology, UNICAMP, 13084-971 Campinas (SP), Brazil. mlsmello@unicamp.br |
| |
Abstract: | The incidence of apoptosis and nuclear instability, including the incidence of catastrophic death, were investigated in benzo[a]pyrene (BP)-transformed human breast epithelial cells (BP1-E cell line) after microcell-mediated transfer of chromosomes 11 and 17. Since the introduction of normal chromosomes 11 and 17 into tumorigenic human breast BP1-E cells reverts some of these cells' characteristics (especially those affected by microsatellite instabilities and loss of heterozygosity) to those of parental non-transformed MCF-10F cells, it was expected that the cell death rates would also be affected by this treatment. The transfer of the mentioned chromosomes, especially chromosome 17, to tumorigenic BP1-E cells increased the apoptotic ratios and decreased the nuclear instability ratios, thus showing that the microsatellite instability and loss of heterozygosity induced by BP in these chromosomes of MCF-10F cells affect the control of cell death mechanisms. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|