Preparation of a stable folate-sepharose complex for affinity chromatography. |
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Authors: | C Fischer S P Rothenberg M da Costa |
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Institution: | Division of Hematology/Oncology, Department of Medicine, New York Medical College, New York, New York 10029 USA |
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Abstract: | A stable folic acid affinity gel has been developed for the purification of nanograms of protein that bind folic acid or its derivatives. The affinity gel was prepared by first coupling folic acid covalently to bovine serum albumin, followed by covalent coupling of the albumin to p-benzoquinone-activated Sepharose. After the albumin-folic acid complex was formed, it was treated with charcoal to remove ionically bound folate which would otherwise elute from the gel and decrease the recovery of the binding protein. The p-benzoquinone activation resulted in a more stable binding of the albumin to the Sepharose. |
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