酵母浸出粉在奈瑟脑膜炎双球菌培养中的比较观察

目的探索A群,C群奈瑟脑膜炎双球菌(简称流脑)多糖疫苗生产中奈瑟脑膜炎双球菌培养的最适培养基。方法在培养基配制中用增减酵母浸出粉的方法制备相应的培养基,8h收菌,通过菌体的收获量并参考多糖量来确定较好的培养基配比。结果不同培养基用于A群、C群奈瑟脑膜炎菌培养8h后均有收获,其中2号培养基(酵母浸出粉)培养的菌体的浓度明显高于1号和3号培养基,它们之间有显著性差异(P<0.05)此种培养基能提高奈瑟脑膜炎双球菌的产量。结论添加酵母浸出粉的培养基可作为A群、C群奈瑟脑膜炎双球菌培养的最适培养基。

Observation on effect of yeast extract in the medium for culture of Neisseria menigitids

Objective To explore the optimal medium for culture of group A and group C Neisseria menigitids in production of Meningococas polysaccharide vaccine.Methods The yeast extract was changed by adjusting its content in the tested medium,the Neisseria meningitids was cultivated for eight hours and then harvested,the best formulation of the medium was selected in comparison of obtained amount of bacterial cells and polysaccharides.Results The obtained amount of bacterial cells was apparently higher in the medium with improved yeast extraction powder(NO.2 medium)by comparing those cultured from NO.1 medium and NO.3 medium,there was a significant deference in NO.2,NO.1,and NO.3 medium(P<0.05).Conclusion NO.2 medium was selected as the optimal formulation used in cultivation of group A and group C Neisseria meningitids.