New crystal forms of ribulose-1,5-bisphosphate carboxylase/oxygenase from Rhodospirillum rubrum |
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| Authors: | G Schneider C I Br?ndén G Lorimer |
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| Affiliation: | 2. University of Tennessee, Knoxville, TN, United States;3. INRS—Institut Armand-Frappier, Université du Québec, Laval, QC, Canada;4. University of Pittsburgh, Pittsburgh, PA, United States;5. Oak Ridge National Laboratory, Oak Ridge, TN, United States;2. Center for Biological Systems Engineering, Washington University School of Medicine, St. Louis, MO, United States;1. Pharmaceutical Chemistry I, Institute of Pharmacy, University of Bonn, 53119 Bonn, Germany;2. Leibniz Institute on Aging, Fritz Lipmann Institute, 07745 Jena, Germany;3. Institute of Physical and Theoretical Chemistry, University of Bonn, 53115 Bonn, Germany;4. Center for Sepsis Control and Care (CSCC), Jena University Hospital, 07747 Jena, Germany;5. Leibniz Institute of Photonic Technology, 07745 Jena, Germany;6. Centre National de la Recherche Scientifique (CNRS), Bioénergetique et Ingenierie des Protéines, UMR 7281, 13009 Marseille, France;7. Institute of Physical Chemistry and Abbe Center of Photonics, Friedrich Schiller University Jena, 07743 Jena, Germany |
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| Abstract: | Three crystal forms of the dimeric form of the enzyme ribulose-1,5-bisphosphate carboxylase from the photosynthetic bacterium Rhodospirillum rubrum have been obtained from the gene product expressed in Escherichia coli. Form A crystals formed from the quaternary complex comprising enzyme-activator carbamate-Mg2+-2'-carboxyarabinitol-1,5-bisphosphate are shown here to be devoid of ligands. In contrast, crystals of the quaternary complex formed with the hexadecameric L8S8 enzyme from spinach contain both the activator carbamate and 2'-carboxyarabinitol-1,5-bisphosphate. Form B crystals of the R. rubrum enzyme are monoclinic, space group P2(1) with cell dimensions a = 65.5 A, b = 70.6 A, c = 104.1 A and beta = 92.1 degrees, with two subunits per asymmetric unit. Rotation function calculations show a non-crystallographic 2-fold axis perpendicular to the monoclinic b-axis. Form C crystals are orthorhombic (space group P2(1)2(1)2(1)) with cell dimensions a = 79.4 A, b = 100.1 A and c = 131.0 A. The monoclinic crystal form diffracts to at least 2.0 A resolution on a conventional X-ray source. |
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