Gas chromatographic determination of the hypoglycaemic agent gliclazide in plasma |
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Authors: | Tadao Maeda Toshikazu Yamaguchi Masahisa Hashimoto |
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Institution: | Department of Analysis, Research Laboratories, Dainippon Pharmaceutical Co., Ltd., 33-94, Enoki-cho, Suita, Osaka 564 Japan |
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Abstract: | A gas chromatographic method has been developed that permits the accurate and specific determination of the hypoglycaemic agent gliclazide in plasma. Gliclazide is extracted with chloroform and, after clean-up, derivatized with diazomethane followed by heptafluorobutyric anhydride to form N-methyl-N′-heptafluorobutyrylgliclazide, which is assayed on a gas chromatograph equipped with a flame ionization detector, an electron-capture detector or a nitrogen—phosphorus sensitive detector.Accurate determinations are possible with flame ionization detection over a concentration range of 1–15 μg/ml of gliclazide in plasma with a relative standard deviation of 5.2%. The minimum detectable concentration with electron-capture detection is 0.02 μg per sample. Plasma levels of gliclazide in dogs following single oral administration (40 mg per dog) have also been determined. |
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