Design and expression of oligomeric fibronectin fusion protein: a strategy
for enhancing cell adhesion activity |
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Authors: | Chong-Pyoung?Chung Email author" target="_blank">Jun-Hyeog?JangEmail author |
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Institution: | (1) Intellectual Biointerface Engineering Center and Department of Periodontology, Seoul National University College of Dentistry, Seoul, 110-768, Korea;(2) Department of Biochemistry, Inha University College of Medicine, Incheon 400-712, Jung-Gu, Korea |
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Abstract: | Cell adhesion to extracellular matrices, including fibronectin, results in clustering of integrins in focal adhesions. To promote the clustering of fibronectin and thus enhance its activity at the sites of focal adhesion formation, we have engineered a fusion protein containing recombinant fibronectin fragment (hFN) connected to the tetramerization helix domain of lac repressor for oligomeric assembly. Purified Lac-hFN fusion protein exhibited significant increase of cell adhesion and proliferation of GF cells compared with hFN alone (p < 0.05). |
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Keywords: | cell adhesion extracellular matrix fibronectin fusion lac repressor |
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