Differential replication of ribosomal DNA during larval development in Calliphora (Diptera) |
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Authors: | J A Thomson |
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Institution: | Department of Genetics, University of Melbourne, Parkville, Victoria 3052, Australia |
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Abstract: | The hybridization of ribosomal RNA with DNA extracted from whole insects has been compared at six stages during the development of the blowfly Calliphora stygia. Total DNA from hatching larvae contains approximately 20% more rDNA than does total DNA from either mid-embryonic or adult insects. DNA from late third-instar larvae close to pupariation contains about 30% less rDNA than that from hatching larvae. The proportion of rDNA to total DNA in larvae of intermediate ages falls between these two extremes. Ribosomal RNA cistrons are thus replicated more rapidly than the remainder of the genome in the late embryonic or earliest larval stages, but more slowly during the later cycles of DNA synthesis in those larval tissues which develop high levels of polyteny. |
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