Restriction cleavage maps of the DNAs of Streptococcus pneumoniae bacteriophages containing protein covalently bound to their 5' ends |
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Authors: | Rubens Ló pez, Concepció n Ronda, Pedro Garcí a, Cristina Escarmí s Ernesto Garcí a |
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Affiliation: | (1) Instituto de Inmunología y Biología Microbiana, C.S.I.C., Velázquez, 144 Madrid 6, Spain;(2) Instituto de Biologia Molecular, C.S.I.C., U.A.M., Universidad Autonoma, Canto Blanco, Madrid 34, Spain |
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Abstract: | Summary Several pneumococcal bacteriophages showing a morphology similar to that previously described for Cp-1 (Ronda et al. 1981) have been isolated and purified from throat samples taken from healthy children. Three of these phages (Cp-5, Cp-7 and Cp-9) have been studied in detail and compared to Cp-1. The four phages differed in several respects, e.g. size, structural polypeptides, restriction enzyme cleavage patterns, etc. The DNA of Cp-5, Cp-7 and Cp-9 showed protease-sensitive transfecting activity. This, together with the results obtained by electrophoretic analyses as well as by isotopic labelling of these DNAs with [-32P] ATP and polynucleotide kinase indicated that all these new phages have a protein covalently linked to the 5 ends of their DNAs as in the case of Cp-1 (García et al. 1983). Restriction enzyme cleavage maps of Cp-1, Cp-5, Cp-7 and Cp-9 have been constructed. |
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