| 反胶束萃取血红蛋白的研究 |
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| 引用本文： | 张亦飞,韩冬冰.反胶束萃取血红蛋白的研究[J].生物技术,1998,8(1):14-17,6. |
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| 作者姓名： | 张亦飞 韩冬冰 |
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| 作者单位： | 烟台大学化学工程系 烟台264005
(张亦飞，韩冬冰，王立新)，烟台大学化学工程系 烟台264005(王学艳) |
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| 摘 要： | 研究了ＣＴＡＢ－正辛醇－正庚烷交束溶液萃取牛血红蛋白（ｐＨｂ）时、ｐＨ值、表面活性剂浓度、助表面活性剂浓度、离子种类和离子强度、溶剂比以及蛋白质浓度等因素对萃取效果的影响，并以蛋白质分子与表面活性剂分子间的相互作用以及反胶束大空间阻碍作用上进行了解释。研究表明，水相ＰＨ值在１０．５￣１２．５之间，ＫＣ１浓度为０．１ｍｏｌ／ｌ，反胶束溶液中表面活性剂浓度为０．０２ｍｏｌ／ｌ，正辛醇与正庚烷之比为０．
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| 关 键 词： | 反胶束溶液 溶剂萃取 血红蛋白 |
Study of BH_b Extraction by Reversed Micelles |
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| ZHang Yifei Han Dongbing Wang Lixin Wang Xueyan.Study of BH_b Extraction by Reversed Micelles[J].Biotechnology,1998,8(1):14-17,6. |
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| Authors: | ZHang Yifei Han Dongbing Wang Lixin Wang Xueyan |
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| Institution: | Department of Chemical Engineering.yantat Vtimfersity.yantat.264005 |
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| Abstract: | BHb extraction by reversed micelles using surfactant CTAB has been studied. Vari-ous parameters of extraction process, such as the PH value, the species and concentration of ion in aqueous phase, the content of surfactant and cosurfactant in reversed micellar solu-tion, and solvent ratio were researched. 97 of BHb in aquous phase was transfered into re-versed micellar solution with one extraction-stripping stage under optimum conditions. The experimental data can be interpreted reasonably from the point of the electrostatic interaction between the Drotein and surfactant, and of the size exclusion effect in extraction nroce |
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| Keywords: | Reversed miceiiar solution solvent extraction BH : CTAB |
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