| 一种快速有效纯化DNA序列分析模板的方法 |
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| 引用本文： | 曾宪春 罗锋. 一种快速有效纯化DNA序列分析模板的方法[J]. 生物技术, 1998, 8(5): 44-45,F003 |
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| 作者姓名： | 曾宪春 罗锋 |
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| 作者单位： | 武汉大学生命科学学院!武汉430072 |
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| 基金项目： | 国家重点科技（攻关）计划项目，武汉市科委青年科技展光计划资助 |
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| 摘 要： | 介绍一种ＤＮＡ序列分析模板的快速、有效的纯化方法。该法对ＤＮＡ模板的回收率可达９５％以上。多次测序结果表明，此法与其他常规纯化方法相比，具有简便、快速、有效、可靠等优点，其测序结果电泳带清晰，无模糊带及“鬼带”出现，重复性及稳定性较好。
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| 关 键 词： | DNA 序列测定 测序模板纯化 |
A EFFICIENT AND RAPID PURIFICATION METHOD OF TEMPLATE DNA FOR DNA SEQUENCING |
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| Zeng Xianchun, Zhu Fan, Jiang Dahe, Luo Feng, Li Wenxin. A EFFICIENT AND RAPID PURIFICATION METHOD OF TEMPLATE DNA FOR DNA SEQUENCING[J]. Biotechnology, 1998, 8(5): 44-45,F003 |
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| Authors: | Zeng Xianchun Zhu Fan Jiang Dahe Luo Feng Li Wenxin |
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| Abstract: | A rapio and efficient puriflcation method of template DNA for DNA sequencing using sephadex G - 50 spun -column is described. The recovery ratio of the plasmid DNA is extimated over 95%. The plasmid purined by this metbod can be used as template foT DNA sequencing directly. lt has clear bands and no appearance of fuzzy and "ghost" bands on polyacrylamide gel in all tests of DNA sequencing. But other purincation mthods don't always obtain this effect. |
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| Keywords: | DNA sequencing DNA purification method of template DNA for sequencing |
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