| 组织型纤溶酶原激活剂突变体细胞t—PA基因拷贝数测定及分析 |
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| 引用本文： | 陈琳 徐秀英. 组织型纤溶酶原激活剂突变体细胞t—PA基因拷贝数测定及分析[J]. 生物技术, 1998, 8(2): 16-18 |
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| 作者姓名： | 陈琳 徐秀英 |
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| 摘 要： | 组织型纤溶酶原激活剂（t—PA）溶栓特异性高，但半衰期短。本组构建了增强纤维蛋白亲合力和延长半衰期的t—PA突变体表达细胞株。测定工程细胞株基因拷贝数是细胞生物学特性鉴定的一个方面。我们采用狭缝杂交法和SouthernBlot法对该细胞株t—PA基因进行了拷贝数测定，结果为30—60个拷贝。
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| 关 键 词： | t—PA 突变体 拷贝数 |
The Determination of Copy Number for Tissue-type Plasminogen Activator(t-PA) Gene |
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| Chen Lin ,Xu Xiuying, Liu Shihui, Zhu Hengqi, Huang Peitang. The Determination of Copy Number for Tissue-type Plasminogen Activator(t-PA) Gene[J]. Biotechnology, 1998, 8(2): 16-18 |
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| Authors: | Chen Lin Xu Xiuying Liu Shihui Zhu Hengqi Huang Peitang |
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| Abstract: | As a thrombolytic drug, t-PA has been used in clinical treatment, but its halflife is so short that the cost is expensive. In ordei to prolong its half-life and increase affinity to fibrin. we have constructed t -PA mutant and obtained expression in CHO -dhfr-cell.We determine the copy number of t -PA gene for the cell line. The results show the copy mumber of t-PA gene is 30-60 copies |
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| Keywords: | t-PA mutant Copy number |
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