Small, stable shuttle vectors based on the minimal pVS1 replicon for use in gram-negative, plant-associated bacteria |
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Authors: | Heeb S Itoh Y Nishijyo T Schnider U Keel C Wade J Walsh U O'Gara F Haas D |
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Affiliation: | Laboratoire de Biologie Microbienne, Université de Lausanne, Switzerland. |
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Abstract: | The minimal replicon of the Pseudomonas plasmid pVS1 was genetically defined and combined with the Escherichia coli p15A replicon, to provide a series of new, oligocopy cloning vectors (5.3 to 8.3 kb). Recombinant plasmids derived from these vectors were stable in growing and nongrowing cells of root-colonizing P. fluorescens strains incubated under different environmental conditions for more than 1 month. |
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