Comparison of different hepatocyte cell lines for use in a hybrid artificial liver model |
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Authors: | M Takagi N Fukuda T Yoshida |
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Institution: | (1) International Center for Biotechnology, Osaka University, 2-1, Yamada-oka, Suita, Osaka 565, Japan |
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Abstract: | Selection of a cell line suitable for a hybrid artificial liver model employing cellulose porous beads (CPBs) was investigated.
Hep G2 cells grown in a culture dish exhibited appreciably higher ureogenesis and gluconeogenesis activities than those grown
in CPBs. SEM observation of CPBs revealed marked difference in the distribution of attached cells from one bead to another,
and showed that almost all the cell-bearing micropores were completely packed with cells.
With the aim of selecting a cell line not prone to excessive aggregation and which grows moderately so as not to fill up the
micropores, cells of 6 cell lines, HLE, HLF, Hep 3B, PLC/PRF/5, Huh 7 and Hep G2, were cultivated in dishes. Hep G2, HLE,
and HLF increased to 5 × 105 cells/cm2, whereas PLC/PRF/5 grew only to 5 × 104, and Hep 3B and Huh 7 up to 2 × 104 cells/cm2. The specific activities of ureogenesis and gluconeogenesis of Huh 7 were the highest among the lines tested - 42- and 7-fold
those of Hep G2, respectively. When the 6 cell lines were grown in a submerged culture with 0.6 g/l of CPBs, Huh 7 had the
lowest cell concentration of 0.54 × 106 cells/ml, and the highest activities of ammonia consumption and urea and glucose production (1.38 μ mol NH3, 99 nmol urea, and 14.5 nmol glucose/106cells/h). Consequently, Huh 7 is considered to be a suitable cell line for use in the development of an artificial liver model
employing porous beads.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | artificial liver gluconeogenesis hepatocyte cell line microcarrier culture porous beads ureogenesis |
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