Advances in quantitative proteomics using stable isotope tags |
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Authors: | Mark R. Flory Timothy J. Griffin Daniel Martin Ruedi Aebersold |
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Affiliation: | Institute for Systems Biology, 1441 North 34th Street, Seattle, WA 98103-8904, USA |
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Abstract: | A great deal of current biological and clinical research is directed at the interpretation of the information contained in the human genome sequence in terms of the structure, function and control of biological systems and processes. Proteomics, the systematic analysis of proteins, is becoming a critical component in this endeavor because proteomic measurements are carried out directly on proteins – the catalysts and effectors of essentially all biological functions. To detect changes in protein profiles that might provide important diagnostic or functional insights, proteomic analyses necessarily have to be quantitative. This article summarizes recent technological advances in quantitative proteomics. |
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Keywords: | proteome profile isotope dilution ICATTM quantitative mass spectrometry liquid chromatography |
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