Cloning and nucleotide sequence of the Enterobacter aerogenes signal peptidase II (lsp) gene. |
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| Authors: | L Isaki M Kawakami R Beers R Hom and H C Wu |
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| Institution: | Department of Microbiology, Uniformed Services University of the Health Sciences, Bethesda, Maryland 20814-4799. |
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| Abstract: | In Escherichia coli, prolipoprotein signal peptidase is encoded by the lsp gene, which is organized into an operon consisting of ileS, lsp, and three open reading frames, designated genes x, orf-149, and orf-316. The Enterobacter aerogenes lsp gene was cloned and expressed in E. coli. The nucleotide sequence of the Enterobacter aerogenes lsp gene and a part of its flanking sequences were determined. A high degree of homology was found between the E. coli ileS-lsp operon and the corresponding genes in Enterobacter aerogenes. Furthermore, the same five genes which constitute an operon in E. coli were found in Enterobacter aerogenes in the same order. |
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