| SCE诱导MCF-7细胞凋亡及其相关基因表达的研究 |
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| 引用本文： | 张敏,陈莉,徐瑞成. SCE诱导MCF-7细胞凋亡及其相关基因表达的研究[J]. 中国组织化学与细胞化学杂志, 2005, 14(5): 525-530 |
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| 作者姓名： | 张敏 陈莉 徐瑞成 |
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| 作者单位： | 武警医学院细胞生物与医学遗传教研室,天津,300162 |
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| 摘 要： | 目的观察鲨鱼软骨提取物(SCE)对MCF-7细胞凋亡的诱导作用,并进一步研究在此过程中BCL-2和Caspase-3的表达.方法将不同浓度的SCE作用于MCF-7细胞,观察其作用的时间效应及剂量效应;在光镜和电镜下观察其形态变化;用流式细胞术分析细胞DNA含量的改变;用免疫组织化学法观察在此过程中BCL-2和Caspase-3的表达.结果 SCE 能抑制MCF-7细胞生长,在一定剂量和时间范围内引起细胞凋亡,并显示剂量和时间效应, 在此过程中BCL-2蛋白表达减弱.结论 SCE 能明显抑制MCF-7细胞体外生长,并可诱导MCF-7细胞凋亡,其作用机制可能与下调Bcl-2蛋白的表达水平有关.
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| 关 键 词： | 鲨鱼软骨提取物 MCF-7细胞系 细胞凋亡 |
| 收稿时间： | 2004-11-12 |
| 修稿时间： | 2005-05-20 |
APOPTOSIS OF MCF-7 CELL LINE INDUCED BY SCE AND EXPRESSIONS OF ASSOCIATED GENES |
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| Zhang Min,Chen Li,Xu Ruicheng. APOPTOSIS OF MCF-7 CELL LINE INDUCED BY SCE AND EXPRESSIONS OF ASSOCIATED GENES[J]. Chinese Journal of Histochemistry and Cytochemistry, 2005, 14(5): 525-530 |
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| Authors: | Zhang Min Chen Li Xu Ruicheng |
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| Abstract: | Objective To observe the apoptosis of the MCF-7 cell line induced by shark cartilage extract (SCE) and the expressions of BCL-2and Caspase-3 in this process. Methods Different concentrations of SCE were used to treat MCF-7 cells, and theinhibition of proliferation was measured by MTT assay. Cell morphology was observed under light and electron microscope. Flow cytometry wasused to analyze DNA contents. The expressions of BCL-2and Caspase-3 were studied by immunohistochemistry. Results SCE could inhibit MCF-7 cell growth.Within a certain range of treating time and dose, apoptosis was induced in a time-and dose-dependent related manner.The expression of BCL-2 was decreased(P<0.01). Conclusion SCE can inhibit the growth of MCF-7 significantly and induce the apoptosis of MCF-7. The decrease of Bcl-2 plays an important role in theprocess. |
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| Keywords: | Bcl-2 Caspase-3 |
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