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Synthesis of a compound soluble in organic solvents from deoxycytidine triphosphate in permeabilized normal human lymphocytes |
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| Authors: | José Mordoh Bertold Fridlender |
| Institution: | (1) Instituto de Investigaciones Bioquímicas  Fundación Campomar , Obligado 2490, Buenos Aires, Argentina;(2) Argentina and Department of Cancerology of the Centro de Investigaciones Médicas Albert Einstein, Luis Viale 2831, Buenos Aires, Argentina;(3) Present address: Virology Department, The Hebrew University, Hadassah Medical School, Jerusalem, Israel |
| Abstract: | Summary Normal human lymphocytes may be rendered permeable to deoxynucleoside triphosphates. When 3H]dCTP is furnished to permeabilized lymphocytes two compounds are formed: DNA and a compound soluble in organic solvents. 3H]dCTP incorporation is higher in stimulated lymphocytes than in unstimulated cells. Some characteristics of the system are reported.Abbreviations PHA Phytohemagglutinin - dNTP deoxyribonucleotide triphosphate - MEM minimal essential medium - PBS Phosphate buffered saline - SSC standard saline citrate This paper is dedicated to Dr.Luis F. Leloir on occasion of his 70th birthday.To whom reprints should be requested. |
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