Immunohistochemical localization of IGF-I,IGF-II and MSTN proteins during development of triploid sea bass (Dicentrarchus labrax) |
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| Authors: | G Radaelli C Poltronieri C Simontacchi E Negrato F Pascoli A Libertini D Bertotto |
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| Institution: | 1.Department of Experimental Veterinary Sciences, University of Padua, Italy;2.Institute of Marine Science (ISMAR), Venice, Italy |
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| Abstract: | The cellular localization of IGF-I, IGF-II and MSTN proteins was investigated during ontogenesis of triploid sea bass (Dicentrarchus labrax) by an immunohistochemical approach. The results were compared with those observed in diploids. IGF-I immunostaining was mainly observed in skin, skeletal muscle, intestine and gills of both diploids and triploids. From day 30 of larval life, IGF-I immunoreactivity observed in skeletal muscle, intestine, gills and kidney was stronger in triploids than in diploids. At day 30, triploids exhibited a standard length significantly higher than the one of diploids. Although IGF-II and MSTN immunoreactivity was detectable in different tissues and organs, no differences between diploids and triploids were observed. The spatial localization of IGF-I, IGF-II and MSTN proteins detected in this study is in agreement with previous findings on the distribution of these proteins in diploid larvae and fry. The highest IGF-I immunoreactivity observed in triploids suggests a possible involvement of ploidy in their growth performance.Key words: IGF-I, IGF-II, MSTN, immunohistochemistry, triploid. |
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