Effects of hypophyseal or thymic allograft on thymus development in partially decerebrated chicken embryos: expression of PCNA and CD3 markers |
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| Authors: | M Aita F Benedetti E Carafelli E Caccia N Romano |
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| Institution: | 1.Department of Physiology and Pharmacology “V. Erspamer”, Faculty of Medicine, University “La Sapienza”, Rome, Italy;2.Department of Environmental Science, University of Tuscia, Viterbo, Italy |
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| Abstract: | Changes in chicken embryo thymus after partial decerebration (including the hypophysis) and after hypophyseal or thymic allograft were investigated. Chicken embryos were partially decerebrated at 36–40 h of incubation and on day 12 received a hypophysis or a thymus allograft from 18-day-old donor embryos. The thymuses of normal, sham-operated and partially decerebrate embryos were collected on day 12 and 18. The thymuses of the grafted embryos were collected on day 18. The samples were examined with histological method and tested for the anti-PCNA and anti-CD3 immune-reactions. After partial decerebration, the thymic cortical and medullary compartments diminished markedly in size. Anti-PCNA and anti-CD3 revealed a reduced immunereaction, verified also by statistical analysis. In hypophyseal or grafted embryos, the thymic morphological compartments improved, the anti-PCNA and anti-CD3 immune-reactions recovered much better after the thymic graft, probably due to the thymic growth factors and also by an emigration of thymocytes from the same grafted thymus.Key words: hypophysectomy, hypophyseal and thymic allograft, chicken embryonal thymus, PCNA, CD3 markers. |
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